前体对桑黄菌丝生长和胞外多糖合成的影响

Effect of Precursors on Mycelium Growth and Polysaccharide Biosynthesis in Medicinal Mushroom Phellinus igniarius

考察不同类型前体对桑黄菌丝生长和胞外多糖合成的影响,在全合成培养基中添加乳糖前体有利于桑黄菌丝的生长和胞外多糖的合成。采用正交试验优化获得乳糖补加条件:发酵初始补加30g/L乳糖,3L发酵罐发酵168h验证实验获得的桑黄菌丝量最高可达13.18g/L,胞外多糖产量最高可达1.21g/L,比对照组提高了25%。而植物油类前体筛选发现菜籽油能明显促进桑黄菌丝的生长,7L发酵罐中发酵144h桑黄菌丝量最高可达23.77g/L,比对照组提高了207.1%,但抑制胞外多糖的合成。

To promote mycelium growth and polysaccharide biosynthesis in Phellinus igniarius, different groups （sugar and plant oil） of precursors were added to synthetic complete medium and the effects of the kind and amount of separately added sugars and plant oils on Phellinus igniarius mycelium growth and polysaccharide biosynthesis were dealt with. A two-factor, three- level full combination design was used to optimize the conditions for lactose supplement, and the results showed that a maximum mycelium biomass of up to 13.18 g/L and a maximum polysaccharide production of up to 1.21 g/L were achieved after 168 h fermentation in a 3 L fermentor with an initial lactose concentration of 30 g/L, and the increment percent of polysaccharide production was 25% in comparison with the control. Among oils from three plants, including soybean, olive and rapeseed, rapeseed oil most obviously promoted the growth of Phellinus igniarius mycelia, and the maximum mycelium biomass was up to 23.77 g/L after fermentation for 144 h in a 7 L fermentor, 207.1% higher than that obtained from the control, while polysaccharide biosynthesis was inhibited by adding rapeseed oil.