产赤藓糖醇菌株的筛选与鉴定

Screening and Identification of Erythritol-producing Strains

利用含有300g/L葡萄糖的高渗培养基从蜂蜜、花粉、土壤等样品中筛选耐高渗酵母菌,经薄层层析和高效液相色谱分析得到两株产赤藓糖醇且不产甘油的酵母菌,通过高碘酸氧化法筛选出其中赤藓糖醇产量较高的一株菌株E54。菌株E54在含葡萄糖200g/L、酵母膏5g/L的发酵培养基中发酵90h,赤藓糖醇产量为41.1g/L,转化率为22.8%。通过形态观察、生理生化实验、5.8SrDNA序列分析并构建系统进化树,初步鉴定E54为Moniliella acetoabutans(丛梗孢酵母)。

Osmophilic yeasts were isolated from honey,pollen and soil by using the medium containing 30% glucose.Two strains producing erythritol but not producing glycerol were identified by thin layer chromatography（TLC） and high performance liquid chromatography（HPLC）.Strain E54 with high ability to produce erythritol was selected by periodic acid oxidation method.The productivity and conversion rate of erythritol were 41.1 g/L and 22.8% after fermentation for 90 h using strain E54 in the medium containing 20% glucose and 0.5% yeast extract.This strain was identified as Moniliella acetoabutans through the analyses of morphological,physiological and biochemical characteristics,5.8S rDNA sequence and the established phylogenetic tree.