一株牛蒡根际纤维素降解芽孢菌的分离鉴定和发酵分析

Isolation,Identification and Fermentation Analysis of a Cellulose-degrading Bacterium from Arctium lappa L.Rhizosphere

从牛蒡根际分离到一株纤维素降解菌株NP10。该菌是芽孢杆菌,其16SrDNA的Genbank序列号是FJ908093。分批发酵法对NP10进行的分析显示,该菌是一株碱性纤维素降解菌。其滤纸酶(FPA)酶活力在32℃、pH8发酵48h达到最大为18.2U/mL,其羧甲基纤维素酶(CMCase)活力在32℃、pH7发酵36h达到最大,为8.1U/mL。

In this study,a cellulose-degrading bacterium,NP10,was isolated from burdock（Arctium lappa L.） rhizosphere.NP10 was identified to belong to the family of Bacillus sp.and its 16S rDNA Genbank accession number was FJ908093.Meanwhile,NP10 was identified as an alkaline cellulose-degrading strain through batch fermentation.A maximum filter paper activity of 18.2 U/mL was obtained after 48 h fermentation under the conditions of 32 ℃ and initial pH 8,and the optimal fermentation temperature and duration as well as initial pH were 32 ℃,36 h and 7,respectively,for achieving a maximum carboxymethyl cellulose activity of 8.1 U/mL.