泰和乌骨鸡活性肽补血作用研究

Hematopoiesis Activity of Bioactive Peptides from Taihe Black-bone Silky Fowls

目的:探讨泰和乌骨鸡活性肽体内补血作用。方法:利用失血法和骨髓抑制药物氟尿嘧啶注射法联合建立小鼠血虚模型,并测定红细胞(RBC)和血红蛋白(HGB)水平,观察乌骨鸡活性肽及其一种分离活性肽(简称组分A)对血虚小鼠的补血作用。结果:活性肽组RBC水平在第12天时与血虚组相比差异显著(P<0.05);活性肽组在第6至12天期间升高RBC和HGB作用水平与阿胶组相比明显加快,具有显著性差异(P<0.05)。第18天时活性肽组HGB水平已超过各组水平,且与正常组相比具有显著性差异(P<0.05)。活性肽组分A组的RBC水平在各个时间点均高于血虚组,但均无显著性差异(P>0.05),活性肽组分A组在第20天时HGB水平已与正常组无显著性差异(P>0.05),而血虚组和阿胶组仍与正常组有极显著差异(P<0.01)。结论:泰和乌骨鸡活性肽及活性肽组分A能够在一定程度上提高氟尿嘧啶血虚模型小鼠的红细胞和血红蛋白水平,具有一定的补血作用。

Objective：To investigate the hematopoiesis activity of bioactive peptides from Taihe Black-bone Silky Fowls （TBSF）.Methods：Blood loss combined with myelosuppression drug （Fluorouracil,5-FU） was used to establish a blood deficiency mouse model.Red blood cells （RBC） and hemoglobin （HGB） levels were measured to evaluate the hematopoiesis effects of bioactive peptides from TBSF and isolated bioactive peptide fraction A.Results：Compared with the blood deficiency group,the RBC level of bioactive peptides group has a significant difference on the 12th day administration （P〈0.05）;During the period of 6 to 12 days,RBC and HGB levels of bioactive peptides group exhibited a significant faster than the Ejiao group （P0.05）.On the 18th day,HGB level of bioactive peptides group was higher than other groups,and also significantly higher than the normal control group （P〈0.05）.The RBC level in bioactive peptide fraction A group was higher than the blood deficiency group at each time point although no significant difference was observed （P〈0.05）,while the blood deficiency group and the Ejiao group exhibited a significant difference （P〈0.01）,compared with the normal control group.On the 20th day,there was no significant difference in HGB level （P〉0.05） between the bioactive peptide fraction A group and the normal control group,while the blood deficiency group and the Ejiao group revealed a significant difference from the normal control group （P〈0.01）.Conclusion：Bioactive peptides from TBSF exhibit hematopoiesis activity by elevating RBC and HGB levels to some extent.