苦瓜伤流液对链脲佐菌素诱导糖尿病小鼠的降糖功能研究

Antihyperglycemic Function of Momordica charantia L.Bleeding sap in STZ-Induced Diabetic Mice

研究苦瓜伤流液(Momordica charantia L.Bleeding Sap,MBS)对链脲佐菌素(STZ)诱导糖尿病小鼠的降血糖功能并探索其作用机理。选用ICR小鼠60只,随机抽取10只作空白对照,余下50只禁食17～18h后腹腔注射STZ170mg/(kgbw·d)。取血糖值在11.1mmol/L以上者随机分组,共3组:模型组、二甲双胍组(200mg/(kgbw·d))和MBS组(200mg/(kgbw·d))。连续灌胃30d后,测定小鼠空腹血糖、血清胰岛素、血脂水平及肝糖原含量。解剖取胰腺,采用石蜡包埋、HE染色,做组织切片观察。结果显示:苦瓜伤流液能显著降低糖尿病小鼠的空腹血糖值及血脂水平,增加血清胰岛素和肝糖原含量;胰腺切片提示,苦瓜伤流液能够改善胰岛组织功能,促进胰岛β细胞修复。结果表明苦瓜伤流液能促进糖尿病小鼠受损胰岛β细胞修复、刺激胰岛素分泌、增加肝糖原合成、调节血脂代谢,从而起到降低血糖的作用。

The present study was aimed at studying the antihyperglycemic effect and its mechanism of Momordica charantia L.bleeding sap （MBS） on streptozotocin-induced diabetic mice.Ten mice were selected randomly as control,while another 50 mice were injected with STZ 170 mgkg bw after fasting for 17-18 h to induce diabetes.The diabetic mice were divided into 3 groups：model group,metformin group （200 mg/（kg bw·d）） and MBS group （200 mg/（kg bw·d））.After 30 days of gavage administration,the levels of fasting blood glucose and blood-fat,serum insulin and hepatic glycogen content were examined.The paraffin sections of pancreas,stained with HE,were made into pathological sections for observation.The results showed that MBS could significantly reduce the levels of fasting blood glucose and blood-fat in diabetic mice,with an increase in hepatic glycogen and insulin content.The pathological sections showed that MBS improved the function of pancreas islet tissues,accelerate the renovation of pancreatic β cells in diabetic mice.The above results indicate that MBS can accelerate the repair of pancreatic inlet β cells injured by streptozotocin,stimulate the secretion of insulin,increase the synthesis of hepatic glycogen and regulate blood lipid metabolism so as to reduce blood glucose level of diabetic mice.