摘要
目的:研究覆盆子糖蛋白的体内外抗氧化作用。方法:通过测定覆盆子糖蛋白粗提物的总还原能力及对羟自由基(·OH)、超氧阴离子自由基(O-2·)和DPPH自由基的清除作用,证明覆盆子糖蛋白粗提物的体外抗氧化作用;并通过测定小鼠血清、肝脏、脑组织中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性的变化,研究覆盆子糖蛋白粗提物对小鼠体内抗氧化性的影响。结果表明:覆盆子糖蛋白粗提物可显著增强小鼠血清、肝脏、脑组织中CAT、SOD、GSH-Px活性;并有一定的还原能力,并可有效地清除·OH、O-2·和DPPH自由基。结论:覆盆子糖蛋白具有明显的抗氧化作用。
Objective:To investigate the antioxidant activity of raspberry glycoprotein (RGP) in vitro and in vivo.Methods:The antioxidant activity of raspberry glycoprotein in vitro was evaluated by determining its reducing power and scavenging capacities against hydroxyl,superoxide anion and DPPH free radicals.The activities of CAT,SOD and GSH-PX in serum,liver and brain tissues of mice were determined to evaluate the antioxidant activity of raspberry glycoprotein in vivo.Results:Raspberry glycoprotein significantly increased the activities of CAT,SOD and GSH-PX in serum,liver and brain tissues of mice,revealed strong reducing power,and effectively eliminated hydroxyl,superoxide anion and DPPH free radicals.Conclusion:Raspberry glycoprotein has obvious antioxidant effect.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第21期357-360,共4页
Food Science
关键词
覆盆子
糖蛋白
自由基
抗氧化
raspberry
glycoprotein
free radical
antioxidation