覆盆子糖蛋白的抗氧化作用

Antioxidant Effect of Raspberry Glycoprotein

目的:研究覆盆子糖蛋白的体内外抗氧化作用。方法:通过测定覆盆子糖蛋白粗提物的总还原能力及对羟自由基(·OH)、超氧阴离子自由基(O-2·)和DPPH自由基的清除作用,证明覆盆子糖蛋白粗提物的体外抗氧化作用;并通过测定小鼠血清、肝脏、脑组织中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性的变化,研究覆盆子糖蛋白粗提物对小鼠体内抗氧化性的影响。结果表明:覆盆子糖蛋白粗提物可显著增强小鼠血清、肝脏、脑组织中CAT、SOD、GSH-Px活性;并有一定的还原能力,并可有效地清除·OH、O-2·和DPPH自由基。结论:覆盆子糖蛋白具有明显的抗氧化作用。

Objective：To investigate the antioxidant activity of raspberry glycoprotein （RGP） in vitro and in vivo.Methods：The antioxidant activity of raspberry glycoprotein in vitro was evaluated by determining its reducing power and scavenging capacities against hydroxyl,superoxide anion and DPPH free radicals.The activities of CAT,SOD and GSH-PX in serum,liver and brain tissues of mice were determined to evaluate the antioxidant activity of raspberry glycoprotein in vivo.Results：Raspberry glycoprotein significantly increased the activities of CAT,SOD and GSH-PX in serum,liver and brain tissues of mice,revealed strong reducing power,and effectively eliminated hydroxyl,superoxide anion and DPPH free radicals.Conclusion：Raspberry glycoprotein has obvious antioxidant effect.