新疆准噶尔铁线莲不同部位多糖提取工艺优化

Optimal Extraction of Polysaccharides from Different Parts of Clematis songarica Bge. in Xinjiang

采用分光光度法,以葡萄糖为标准样品测定新疆准噶尔铁线莲不同部位中的多糖含量,采用正交试验设计对其不同部位中多糖提取工艺进行最佳筛选、优化。分别得到的最佳提取工艺条件为:茎:温度90℃、浸提时间2.5h、料液比1:60(g/mL)、提取次数5次,OD值达0.408;叶:温度90℃、浸提时间2h、料液比1:60(g/mL),提取次数5次,OD值达0.493;花:温度90℃,时间2h,料液比1:60,提取次数4次,OD值达0.418;果实:温度90℃、浸提时间2h、料液比1:60(g/mL)、提取次数5次,OD值达0.315。

Polysaccharides were extracted from different parts of Clematis songarica Bge. and determined by using spectrophotometry method with glucose as the standard. The optimal extraction parameters of polysaccharides were explored by orthogonal experiments to be extraction temperature of 90 ℃, material-liquid ratio of 1： 60, extraction time of 2.5 h and repeated extraction number of 5 for stem; extraction temperature of 90 ℃, material-liquid ratio of 1： 60, extraction time of 2 h and repeated extraction number of 5 for leaves; extraction temperature of 90 ℃, material-liquid ratio of 1： 60, extraction time of 2 h and repeated extraction number of 5 for flowers; extraction temperature of 90 ℃, material-liquid ratio of 1： 60, extraction time 2 h and repeated extraction number of 5 for fruits. The absorbance of polysaccharides extracted from stem, leaves, flowers and fruits at 600 nm were 0.408, 0.493, 0.418 and 0.315, respectively.