超临界CO_2状态下生物酶法催化合成植物甾醇酯

Lipase-catalyzed Synthesis of Phytosterol Ester in Supercritical CO_2

对葵花油与植物甾醇在CO2超临界状态下合成植物甾醇酯的工艺进行研究。采用Novozym435脂肪酶做催化剂,进行酯化反应。通过单因素与正交试验,确定了最佳工艺条件:反应温度85℃、植物甾醇质量分数5%、反应压力8MPa、反应时间1h、搅拌速度600r/min,在此条件下,转换率为92.1%。与常规方法相比,本工艺降低了反应温度,缩短了反应时间。

In the supercritical CO2 condition, the synthesis of phytosterol ester was investigated using sunflower oil and phytosterol as the materials and Novozym 435 lipase as the catalyst. The optimal esterification conditions were achieved through single factor and orthogonal experiments to be reaction temperature of 85 ℃, phytosterol concentration of 5%, reaction pressure of 8 MPa, reaction time of 1 h, stirring speed of 600 r/min, and lipase dosage of 1%. The conversion rate was up to 92.1% under the optimal reaction conditions. This study will provide a theoretical reference for the preparation of phytosterol ester.