荧光分光光度计测定保健食品总抗氧化能力

Total Antioxidant Capacity Determination of Health Food by Fluorescence Spectrophotometer

目的:建立用荧光分光光度计测定总抗氧化能力的方法。方法:以2,2'-偶氮二(2-脒基丙烷)二盐酸盐(AAPH)作为过氧自由基来源,sodium fluorescein(FL)为荧光指示剂,水溶性VE(trolox)为定量标准,荧光值半数衰减时间作为观察指标,测定6种保健食品的总抗氧化能力。结果:方法的最低检测限为0.22μmol/L,线性回归方程Y=25.432X+2.0625,R2=0.9949,平行性精密度(RSD)3.5%,重复性精密度(RSD)3.9%,加标回收率在90%～104%之间。结论:用荧光分光光度计可以测定保健食品总抗氧化能力。

Objective： To establish a determination method of total antioxidant capacity by fluorescence spectrophotometer. Methods： Total antioxidant capacity of health food were determined by a fluorescence method using AAPH as the source of peroxy free radicals, sodium fluorescein （FL） as the fluorescent indicator, water-soluble vitamin E （Trolox） as the quantitative standard and half fluorescence decay time as the observation index. Results： This established method had the detection limit of 0.22 μ mol/L, linear regression equation of Y = 25.432X ＋ 2.0625 with a coefficient constant of 0.9949, parallel precision of 3.5% and repeatability precision of 3.9%. The recovery rates of spiked samples were in the range of 90%-- 104% through the determination of six health food samples. Conclusion： Fluorescence spectrophotometer can be used for total antioxidant capacity of health food.