顶空固相微萃取-气相色谱/质谱法分析霸王花挥发性成分

Analysis of Volatile Components in Pitaya Flower by Headspace Solid Phase Microextraction-GC/MS

目的:探索分析霸王花挥发油化学成分的方法。方法:采用聚二甲基硅氧烷萃取头室温顶空微萃取20min、初始柱温60℃、程序升温至250℃、进样口温度250℃的气相色谱条件,EI离子源、电子能量70eV的质谱条件进行气相色谱/质谱法分析。结果表明:共鉴定出49种化合物,主要挥发性成分及其含量为倍半萜及其含氧衍生物(27.78%)、脂肪族化合物(25.14%)、酯(14.40%)、2,6-二叔丁基对甲酚(10.08%)和硅氧烷类化合物(6.27%)。结论:无溶剂的顶空萃取-气相色谱/质谱联用技术可作为霸王花挥发性成分分析的有效方法。

Objective： To explore the analytic method of volatile components in pitaya flower. Methods： Headspace solid phase microextraction （HS/SPME） technology was used to extract volatile components from pitaya flower. Gas chromatography coupled with mass spectrometry （GC-MS） was used to identify volatile components in pitaya flower at the condition of programmed temperature increase to 250 ~C, sample injection temperature at 250 ℃ and electronic ionization as the ion source. Results： A total of 49 volatile compounds were identified, which included sesquiterpenes and their oxidized derivatives （27.78%）, alkanes （25.14%） and esters （14.40%）, 2,6-di-tert-butyl-p-cresol （10.08%） and siloxane compounds （6.27%）. Conclusion： HS/ SPME-CJC/MS strategy is a useful and feasible method for the analysis of volatile components in pitaya flower.