基质固相分散辅助加速溶剂萃取-气相色谱法测定花生中有机磷农药残留

Determination of Organophosphorus Pesticide Residues in Peanut by Assisted Solid-phase Dispersion Accelerated Solvent Extraction-Gas Chromatography

基质固相分散辅助加速溶剂萃取-气相色谱法测定花生中5种有机磷农药残留。花生样品与硅藻土、弗罗里硅土混合后装填萃取池,加速溶剂萃取仪在10.0MPa、80℃条件下,丙酮作为提取溶剂,加热5min,静态提取5min,循环2次的条件下用气相色谱(氮磷检测器)进行分析。在添加水平为0.05、0.1mg/kg时,花生中5种有机磷农药的加标回收率为88.2%～104.5%,测定结果的相对标准偏差为3.2%～7.1%(n=6),检出限为0.030～0.038mg/kg。该法具有良好的分离效果、较宽的线性关系和较高的灵敏度。

Five organophosphorus pesticide residues from peanut were determined by using solid-phase dispersion-assisted accelerated solvent extraction coupled with gas chromatography. The peanut samples were mixed with florisil and diatomite to fill the extraction instrument. Extraction was performed with acetone at 10.0 MPa and 80 ℃ for heating 5 min and static extraction 5 min. Elution was analyzed with GC-NPD detector. At the spiked sample levels of 0.05 mg/kg and 0.1 mg/kg, the recovery rates of organophosphorus pesticide residues were 88.2% 104.5% with a relative standard deviation less than 7.1% and the detection limit of 0.030 0.038 mg/kg. This method has excellent resolution, high sensitivity, good precision and wide linearity.