采后一氧化氮处理调控番茄果实茉莉酸类物质合成并提高灰霉病抗性

Effect of Postharvest Nitric oxide Treatment on Jasmonates Biosynthesis and Disease Incidence to Botrytis cinerea Pers. in Tomato Fruits

探讨在番茄果实抗病性诱导过程中一氧化氮对茉莉酸类物质的调节作用。以中蔬4号绿熟期番茄果实为材料,对其进行不同浓度一氧化氮处理,检测不同处理对番茄果实抵抗病原菌侵染能力、一氧化氮水平、茉莉酸类物质含量及其关键合成酶活性以及抗病酶苯丙氨酸解氨酶活性的影响。结果表明:不同浓度一氧化氮处理均能提高采后番茄果实抵抗病原菌侵染的能力,低浓度一氧化氮供体硝普钠(0.02mmol/L)表现得更为明显;低浓度硝普钠能够促进JAs含量及脂氧合酶活性的增加,高浓度处理则表现出抑制作用。

Nitric oxide （NO） and jasmonates （JAs） are important signaling molecules in plant disease resistance. Recent studies have shown the evidences about NO cross talks with jasmonic aicd （JA） pathway. In order to find the relationship between NO and JAs during disease resistance induction, tomato fruits （Lycopersicon esculentum cv. No.4 Zhongshu） were treated with different concentration of NO, then measured the effects on resistance to Botrytis cinerea Pers., NO level, JAs content, the key enzyme activity of JA biosynthesis and phenylalnine ammonialyase activity which is related to disease resistance. Our results showed that treatment with NO could induce disease resistance at all NO levels tested, however, the most obvious induction symptom was recorded in low concentration （0.02 mmol/L）. Low concentration treatment increased JAs contents and the key enzyme activity of JA biosynthesis, while inhibited in high concentration treatment.