湖南省肾综合征出血热Ⅰ型灭活疫苗效果免疫(感染)增强和免疫策略研究

Evaluationg on the Efficacy of Vaccines Against HFRS and Study of Antibody Dependent Immunization Enhancement and Immunological Strategy

采用ＩＦＡＴ法检测特异ＩｇＧ荧光抗体，用ＭＣＰＥＮＴ法检测中和抗体滴度，追踪流行病学防病效果。结果表明，该疫苗的安全性较好，总反应率为３１９％，中强反应１８２％，反应以局部一过性疼痛多见，无异常反应。疫苗３针全程免疫后２周、加强前、加强后２周、１年和２年，３年中和抗体阳转率分别为３１４０％、９３３％、８７５０％、５３８５％、３８１０％和３５００％。荧光抗体阳转率分别为８４９１％、２２５０％、８５００％、１５７９％、１０２０％和９０５％。疫苗的流行病学防病效果好，３针全程免疫后（１９９５４）至今（１９９９．３）接种者无人发病，对照组发病８例，保护率为１００００％，观察到试区疫情较四周非试区低，疫苗复盖率达５０％以上，似可有效控制疫情。发现有１例接种者在经过一个流行高峰期后荧光抗体较前呈４周以上增高，但无临床表现。未发现接种人群中有免疫（感染）增强问题。

The purpose of this report was to observe the safety,serological and epidemiological efficacy of Type I inactivated vaccines against HFRS that was purified from mouse brains and supplied by Institute of Biological products,Lanzhou.Methods:Immunoflurescent antibody assay was used in testing specific IgG antibody and Mcro CPE method was used in testing the titer of neutralizing antibody.There is cohort study for epidemiological efficacy.Results:This vaccine was safe.The rate of genernal response was 3 19%,and the rate of medium and strong response was 1 82%.Two weeks after three doses of infection,before reinforced,two weeks,one year and two years after reinforced.The rates of seroconversion of neutralizing antibody were 31 14%,9 33%,87 50%,53 85%,35 80% and 30 10% respectively.The rates of seroconversion of immunofluorescent antibody were 84 91%,22 50%,85 00%,15 79%,9 52% and 10 00% respectively.This vaccine has good efficacy in prevention disease.After three doses of injection,there were no patients in the immunized group and eight cases in the control group,so the protection rate was 100%.After the epidemic peak,the inoculated individuals didn't show any clinical symptom,but increase of immunofluorescent antibody was four times more than before.Till now,we haven't find any antibody dependent immunization enhancement phenomenon among the inoculated population.Chonclusion:The type I vaccine (supplied by Institute of Biolocal Products,Lanzou)was safe and epidmiologically effective.We also offered some suggestions on the immunological strategy.