I-Eβ基因与实验性小鼠膜性肾病发病的相关性研究

Correlation of I-Eβ gene and the pathogenesis membranous nephropathy in experiment mouse model

目的探讨小鼠控制免疫应答的基因——Ir基因(immune response gene)I-Eβ片段与膜性肾病(membranous nephropathy,MN)发病机制的关系。方法复制和鉴定小鼠膜性肾病动物模型,以RT-PCR扩增并鉴定I-Eβ基因,PCR产物送北京华大基因研究中心测序。测序结果与DNA序列峰图核对后,以clustalx1.83进行多重序列对比。以杂合突变数进行统计处理。结果 PCR产物测序序列以clustalx1.83进行多重序列对比,结果符合率100%。杂合突变分析显示:实验组突变率为9.179‰;对照组突变率为5.059‰,实验组和对照组差异具有统计学意义。结论 I-Eβ基因在实验性膜性肾病小鼠中杂合突变率增高,可能和小鼠膜性肾病发生有关。

In this study,we aimed to study the relation between the mechanism of membranous nephropathy（MN） and the immune response gene-Ir gene（I-Eβ segment） gene.We reproduced and identified the animal model of MN in mouse;I-Eβ gene was amplified and verified by RT-PCR,then sequenced by Beijing Huada Gene Study Center.The detected sequences were checked with the kurtosis,and contrasted with the normal one by clustalx1.83.Then the heterozygosis mutation was analyzed statistically.We found that the sequence is totally tallied with the normal one（100%）;rate of heterozygosis mutant in experimental group is 9.179‰,while that in control group is 5.059‰,which is different statistically.The heterozygosis mutant rate of I-Eβ in experimental mouse is elevated,which maybe relate to membranous nephropathy.