骨形态发生蛋白9对人乳腺癌MDA-MB-231细胞增殖与凋亡的作用研究

The effects of BMP9 expressions on proliferation and apoptosis of human breast cancer cell line MDA-MB-231

目的:研究骨形态发生蛋白9(Bone morphogenetic protein 9,BMP9)对乳腺癌细胞MDA-MB-231增殖、周期及凋亡的影响。方法:半定量RT-PCR法检测高转移性乳腺癌细胞MDA-MB-231和低转移性乳腺癌细胞MCF-7中BMP9的表达,用人BMP9重组腺病毒(AdBMP9)感染MDA-MB-231细胞作为实验组,含GFP的空载腺病毒(AdGFP)感染MDA-MB-231细胞作为对照组,MTT法观察细胞增殖的变化,流式细胞仪分析周期及凋亡的变化。结果:半定量RT-PCR显示,乳腺癌细胞MDA-MB-231中未检测到BMP9 mRNA的表达;MTT结果显示,AdBMP9处理可引起MDA-MB-231细胞增殖抑制,第5 d MDA-MB-231/BMP9组的细胞增殖率(0.8860±0.0532)显著低于MDA-MB-231/GFP组(1.2240±0.1031)(P<0.05);流式细胞仪分析结果显示,腺病毒感染后第2 d和第3 d,MDA-MB-231/BMP9组细胞周期各相发生变化,G2/M期细胞明显增加,分别为MDA-MB-231/GFP组的3.2倍和2.4倍(P<0.05);同时BMP9可诱发细胞凋亡,腺病毒感染后第3 d MDA-MB-231/BMP9组细胞凋亡百分率(31.55%±8.26%)明显高于MDA-MB-231/GFP组(3.80%±0.46%)(P<0.05)。结论:提示BMP9可通过阻滞细胞周期进程和诱导细胞凋亡来抑制乳腺癌细胞MDA-MB-231的增殖。

Objective：To investigate the effects of bone morphogenetic protein 9 on the proliferation, cell cycle and apoptosis of breast cancer cell line MDA-MB-231.Methods：The expression of BMP9 in high-metastatic cell line MDA-MB-231 and low-metastatic cell line MCF-7 were detected by Semi-quantitative PCR respectively,MDA-MB-231 were transfected with AdBMP9（as experimental groups） and AdGFP（as control groups） respectively,Then the ability of cell proliferation was detected by MTT,cell cycle and apoptosis were detected by flow cytometry.Results：The BMP9 mRNA was not detected in human breast cancer cell line MDA-MB-231.After transfection with AdBMP9,the MDA-MB-231 cells showed an inhibited proliferation,On the fifth day,the growth rate of experimental groups（0.886 0±0.053 2）was significantly lower than that of control groups（1.224 0±0.103 1）（P0.05）;The results of flow cytometry showed that,BMP9 could induce the cell cycle arrest in MDA-MB-231,On the second day and third day after transfection,the cell number of G2/M phase increased significantly,which were 3.2 fold and 2.4 fold that of control groups respectively;It＇s also found that after 72h treatment,the apoptosis rate of experimental groups（31.55%±8.26%） was significantly higher than that of control group（3.80%±0.46%）（P0.05）.Conclusion：These data demonstrated that BMP9 could inhibit the proliferation of MDA-MB-231 by blocking cell cycle and inducing apoptosis.