乌司他丁对A549细胞在缺氧条件下ENaC-α表达影响的研究

Ulinastatin inhibits the action of Hypoxia on ENaC-α mRNA and protein expression

目的:研究乌司他丁对缺氧A549细胞ENaC-α mRNA和蛋白表达水平的影响。方法:将A549细胞分组为缺氧对照组和乌司他丁组,分别在3%O2和0%O2的缺氧条件下,用1 250、2 500、5 000 U/ml浓度乌司他丁作用A549细胞,用RT-PCR半定量ENaC-α mRNA表达量,用Western blot半定量ENaC-α蛋白浓度。结果:在不同缺氧程度下,乌司他丁组的ENaC-α mRNA和蛋白表达均高于对照组,呈浓度依赖方式。结论:乌司他丁能够对抗缺氧对ENaC-α表达的抑制作用,提示乌司他丁在急性肺损伤(Acute lung injury,ALI)的治疗过程中具有缓解肺水肿、减轻肺损伤的作用。

Objective：To study the effect of Ulinastatin on ENaC-α mRNA and protein of human alveolar epithelial A549 cells which are treated with hypoxia.Methods：A549 cells were divided into two groups,hypoxia group and ulinastatin group.A549 cells were treated with Ulinastatin at the doses of 1 250, 2 500 U/ml and 5 000 U/ml for 48 h when cotreated with 3% O2 or 0% O2.The mRNA and protein levels of ENaC-α were determined by semiquantitative RT-PCR and Western blot.Results：Incubation of A549 with 1 250 U/ml,2 500 U/ml and 5 000 U/ml of Ulinastatin for 48 h could significantly upregulate the expression of ENaC-α in a concentration-dependent manner.Conclusion：Ulinastatin could reverse the inhibitory effect of hypoxia on the expression of ENaC-α,which suggests that ulinastatin can promote lung fluid clearance and pulmonary edema reduction.