微淋巴管形成对胃癌淋巴结转移的影响

The relationship between micro lymphangiogenesis and lymphatic metastasis in gastric carcinoma

目的:探讨微淋巴管形成在胃癌淋巴结转移中的作用及其二者间的病理相关性。方法:取40例胃癌组织(筛选出有无淋巴结转移病例各20例,分为有转移组和无转移组),另取15例胃溃疡良性病变组织作对照组,采用免疫组化SP法检测血管内皮生长因子C(Vascular endothelial growth factor-C,VEGF-C)、血管内皮生长因子受体3(Vascular endothelial growth factor re-ceptor-3,VEGFR-3)表达水平,再以肾水平蛋白(Podoplanin)标记微淋巴管内皮细胞,计数微淋巴管密度(Lymphatic microvesseldensity,LMVD)及分布特点。结果:胃癌组与对照组之间LMVD存在显著差异(P<0.05),LMVD在有转移组与无转移组有显著差异(P<0.05),胃癌组中LMVD与性别年龄无显著相关性,VEGF-C表达胃癌组与对照组有显著差异(P<0.05),在有转移组与无转移组间表达强度有显著差异(P<0.05)。VEGF-C表达与VEGFR-3表达有高度相关性,VEGFR-3的表达在有转移组与无转移组间有显著差异(P<0.05)。结论:VEGF-C与VEGFR-3高表达能够促进微淋巴管的生成,微淋巴管的增多亦可增强VEGF-C与VEGFR-3的表达。微淋巴管的增多在促进胃癌淋巴结转移中有重要意义。

Objective：To investigate the formation and the distribution characteristics of the micro lymphatic vessels in the stomach cancer and discuss the pathological relationship between lymphatic node metastasis and micro lymphangiogenesis.Methods：40 cases of gastric cancer tissues were selected ,including 20 cases of lymph node metastasis as metastasis group and 20 cases without lymph node metastasis as non-metastasis group as well as.15 cases of benign gastric ulcer lesions as control group.Immunohistochemical SP method was used to detect the expression level of VEGF-C and VEGFR-3. Then Podoplanin was marked in micro lymphatic endothelial cells in order to count lymphatic micro-vessel density and watch the distribution characteristics of the lymphatic microvessel density in metastasis and non-metastasis groups as compared with the control group.Results：The LMVD in gastric cancer groups was significantly higher than that in control group（P0.05）.The LMVD in metastasis group was significantly higher than that in non-metastasis group（P0.05）.The LMVD in gastric cancer groups had no relationship with gender and ages.The expression of VEGF-C was significantly higher in gastric cancer groups than that in zhe control group（P0.05）.There was significantly difference in the expression of VEGF-C between metastasis group and non-metastasis group（P0.05）.There was significantly high relationship between the expressions of VEGF-C and VEGFR-3.There was significantly difference in the expression of VEGFR-3 between metastasis group and non-metastasis group（P0.05）.Conclusion：The high expression of VEGF-C in the gastric cancer can promote micro lymphangiogenesis.The increase in micro-lymphatic vessels can also enhance the expression of VEGF-C.The above two have positive correlation to each other.The increase of micro-lymphangiogenesis promotes lymph node metastasis of gastric cancer.