人脑胶质瘤组织内微血管定量和nm23基因表达

Microvessel quantitation and nm23 gene expression in human brain glioma

目的：探讨人脑胶质瘤组织内微血管数量（ＭＶＣ）和ｎｍ２３基因表达的临床意义．方法：对５４例人脑胶质瘤组织内的ＭＶＣ和ｎｍ２３基因表达采用免疫组化染色进行回顾性研究．结果：ＭＶＣ随胶质瘤的恶性程度及瘤周水肿的严重程度增加而增多，复发组ＭＶＣ明显高于未复发组（Ｐ＜０．０１）．同时发现ｎｍ２３表达阳性强度与人脑胶质瘤的恶性程度及瘤周水肿呈负相关（ｒ分别为－０．４８和－０．５１，Ｐ＜０．０１），复发组ｎｍ２３阳性表达率显著低于未复发组（Ｐ＜０．０５），且瘤组织内ＭＶＣ与ｎｍ２３表达阳性强度呈负相关（ｒ＝－０．５３，Ｐ＜０．０１）．结论：ＭＶＣ和ｎｍ２３基因表达是两个相关联的、可反映人脑胶质瘤生物学行为的有用指标．

AIM: To investigate the clinical significance of microvessel count (MVC) and expression of nm23 gene in human glioma. METHODS: MVC and expression of nm23 gene in 54 cases of glioma were assessed retrospectively by immunohistochemical staining. RESULTS: MVC increased with the degree of malignance and the gravity of peritumor edema and was significantly higher in the recurrent group than in the nonrecurrent group (P<0.01). Meanwhile, a significant negative correlation between the intensity of nm23 gene expression and the malignant status and peritumoral edema was found (r=-0.48 and -0.51, P<0.01 respectively). nm23 positive expression rate was associated with a lower incidence in the recurrent group than in the diseasefree group (P<0.05). And there was a significant negative correlation between MVC and nm23 protein positive degree in tumor tissues (r=-0.53, P<0.01). CONCLUSION: MVC and expression of nm23 gene are the correlatively useful indicators for the biological behavior of human glioma.