国人细小病毒B19感染相关疾病谱

The disease spectrums related with human parvovirus B19 infection in China

目的：探讨我国人细小病毒Ｂ１９感染引起的相关疾病谱．方法：用聚合酶链反应（ＰＣＲ）技术及原位杂交技术对６６例先天性心脏病（ＣＨＤ）心肌组织标本及１０８例血小板减少性紫癜（ＴＰ）、４７例急性再生障碍性贫血（ＡＡＡ）、５例传染性红斑（ＥＩ）及４０例缺铁性贫血（ＩＤＡ）患儿的外周血、骨髓标本进行了Ｂ１９－ＤＮＡ检测，并随机对其中２３例Ｂ１９－ＤＮＡ阳性标本作巨细胞病毒（ＣＭＶ）、单纯疱疹病毒（ＨＳＶ）、ＥＢ病毒（ＥＢＶ）及弓形体（ＴＯＸ）－ＤＮＡ的检测．结果：①上述疾病中Ｂ１９－ＤＮＡ的总阳性检出率为２６．３％（７０／２６６），其中ＥＩ，ＡＡＡ，ＩＴＰ和ＣＨＤ病例中Ｂ１９－ＤＮＡ的阳性率分别为１００％（５／５），２５．５％（１２／４７）及３８．０％（４１／１０８）和１８．２％（１２／６６）；②２３例Ｂ１９－ＤＮＡ阳性病例中，ＡＡＡ，ＴＰ及ＣＨＤ的标本ＣＭＶ－ＤＮＡ的检出率分别为１０．０％（１／１０），２０．０％（１／５）及４０．０％（２／５）．结论：我国ＨＰＶＢ１９病毒感染可引起ＥＩ，ＡＡＡ和急性血小板减少性紫癜，并可能为ＣＨＤ的重要致病因子之一．

AIM: To explore the disease spectrums related to human parvovirus B19 (HPV B19) infection in China. METHODS: By using nested polymerase chain reaction assay and in situ hybridisation, HPVB19 DNA was detected in cardiac tissue from 66 patients with congenital heart disease (CHD), in peripheral blood and bone marrow from 47 patients with acute aplastic anemia (AAA), 108 with thrombocytopenic purpura (TP), 5 with erythema infectiosum (EI), and 40 with iron deficiency anemia (IDA). Meanwhile, cytomegalovirus (CMV), herpes simplex virus (HSV), EpsteinBarr virus (EBV) and toxoplasma (TOX) were also detected in 23 randomly selected cases of HPVB19 positive. RESULTS: The total positive rate of HPV B19 was 26.3% (70/266). The HPVB19 positive rates in EI, AAA, TP and CHD were 100% (5/5), 25.5% (12/47), 38.0% (41/108) and 18.2% (12/66) respectively. The CMV positive rates in the 23 cases of HPVB19 positive specimens of AAA, TP and CHD were 10.0% (1/10), 20.0% (1/5), 40.0% (2/5) respectively. ZCONCLUSION: HPVB19 infection may cause EI,AAA and TP,and may be one of the pathogens of CHD.