摘要
目的：分别以红藻氨酸（ＫＡ）微注射和γ－刀照射大鼠单侧尾状核，比较两者星形胶质细胞（ＡＳ）的适应性变化．方法：用抗胶质纤维酸性蛋白（ＧＦＡＰ）的ＡＢＣ法检测在受损伤３ｈ至３０ｄ的不同时期ＡＳ的变化．结果：２种损伤方式都可引起靶区ＧＦＡＰ阳性反应的细胞数量的增加，并且都可见到两种类型的阳性细胞，即胞体小、突起细的纤维型和胞体大、突起粗的肥大型．但二者在ＡＳ的反应顺序上有不同，在ＫＡ微注射组，２４ｈ内靶区呈大片坏死，几乎无阳性反应出现，３ｄ后坏死区周围有少量ＡＳ出现，随成活时间延长，ＡＳ的增生和肥大增加；而γ－刀照射组，靶区在３ｈ～７ｄ时都有ＡＳ的增生和肥大，１４ｄ时靶区有坏死点出现，ＡＳ数量比３ｈ～７ｄ时减少，３０ｄ时出现坏死区，且在坏死区周围ＡＳ反应呈层次变化．距离靶区越近，ＡＳ变形越明显，距靶区越远，ＡＳ渐呈现正常细胞形态．结论：γ射线和ＫＡ在损伤大鼠脑组织后可引起星形胶质化反应；ＧＦＡＰ可以作为一种衡量ＣＮＳ损伤的指标；两者反应的不同之处则提示二者的作用机理不同．
AIM: To compare the response of astrocytes when unilateral nucleus caudateputamen in rats were microinjected with kainic acid (KA) or irradiated with gamma knife respectively. METHODS: The astrocytes were identified with antiGFAP immunohistochemical ABC method and the progress of their reaction to the two insults was examined from 3 h to 30 d after the lesion. RESULTS: Both lesions induced a similar hyperplasia and hypertrophy of astrocytes and two types of GFAPir cells were found, one with a small cell body and thin process, and the other with a hypertrophic cell body and thick and long process. Difference existed in the time course of GFAP expression in the two groups. In KA microinjected group, large necrotic area formed in the target within 24 h. Three days later, a few astrocytes appeared around the necrosis. With the increase of the survival time, hyperplasia and hypertrophy of astrocytes began to increase. Whereas in gammaknife irradiated group, hyperplasia and hypertrophy were evident from 3 h to 7 d and necrotic dots could be seen in the target on day 14. On day 30, necrosis was more obvious with gradual variations in GFAP expression around the necrotic area. CONCLUSION: The above results indicated that GFAP can be used as a marker for CNS injury. The timing and distribution pattern difference in both KA microinjection group and gammaknife irradiation group suggests that the microinjection and irradiation have different mechanisms.
出处
《第四军医大学学报》
1999年第6期511-514,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金
