人DNA修复基因hR24L参与细胞周期检定点调控

hR24L, a functional human homologue of S.cerevisiae RAD24L gene, is involved in cell cycle checkpoint control

目的：研究酵母ＲＡＤ２４Ｌ基因功能，克隆人类同源物。方法：分离ＲＡＤ２４基因区域ｒａｄ２４Ｌ突变株，以人的ｃＤＮＡ表达文库转化突变株，筛选回复表型；大剂量Ｘ线照射观察细胞周期。结果：ｒａｄ２４Ｌ对紫外线敏感，从转化文库后的回复表型中克隆出人类ｈＲ２４Ｌ全长ｃＤＮＡ。结论：克隆的人类ＤＮＡ修复基因ｈＲ２４Ｌ，它能校正ｒａｄ２４Ｌ的突变表型，参与细胞周期检定点调控。

Objective:To study the function of RAD24L of S.cerevisiae and clone human functional homologue. Methods:The UV sensitive mutant rad24L was isolated by plasmid shuffling. Then, transformation of rad24L was performed with human cDNA expression library to screen for reversion phenotype. The cell cycle was studied by X ray irradiation. Results:The mutant rad24L was obtained and a full length cDNA was cloned from reversion phenotype by transformation with human expression library. Conclusion:Human functional homologue of RAD24L was cloned. The hR24L which corrected the phenotype of rad24L mutant is involved in cell cycle checkpoint control.