摘要
用百合的O/A系列间杂种为实验材料,通过组织培养,比较不同浓度的秋水仙素及不同处理时间长度的诱导效果的差异。结果表明,用浓度为0.05%的秋水仙素处理24 h的效果最佳,突变率高达38.5%。与正常植株相比,变异植株的叶片肥厚,叶面变宽,叶色深绿,气孔显著增大。通过显微镜观察根尖细胞,发现其染色体数目为2n=4x=48。
Taking lily derived from intersectional hybrids between Oriental hybrids and Asiatic hybrids as experimental materials,the inducing effects of different concentration colchicine and different treating time were compared.The results showed that 0.05 % colchicine and treating time 24 h got the optimum inducing effect,with the inducing percentage of 38.5 %.Compared with the control,the induced plant had thicker and wider leaf,the color of leaf was dark green,and the stoma was increased significantly.Moreover,the cell of root tip was observed under microscope,it was found that the number of chromosome was 2n = 4x = 48.
出处
《西部林业科学》
CAS
2010年第4期82-84,共3页
Journal of West China Forestry Science
基金
国家科技支撑计划项目(2007BAD45B01)
贵州省体改项目[2009]4006
贵州省基金项目[2008]262
贵州省省长基金项目[2008]80
贵州省农业动植物育种专项[2009]031
贵州省高层次人才特助项目TZJF-2008年27号
贵州省农科院专项[2008]032
