ITS序列在桉树种质资源鉴定中的应用研究

Application of ITS Sequences in Eucalyptus Germplasm Identification

利用ITS序列分析技术对3个桉树优良无性系广林巨尾桉5号（GLGU-5）、广林巨尾桉9号（GLGU-9）、广林尾叶桉4号（GLU-4）以及巨尾桉和尾叶桉实生苗材料进行位点差异性分析。并根据ITS序列变异特点对3个优良无性系与巨尾桉和尾叶桉实生苗进行分子鉴定。结果表明：（1）优良无性系的ITS扩增片段比巨尾桉、尾叶桉实生苗长38~45 bp,因此,无性系与尾叶桉、巨尾桉实生苗之间ITS序列的长度差异,可以作为区分无性系与巨尾桉和尾叶桉实生苗之间的依据;（2）无性系之间存在ITS碱基位点差异,这些位点差异可以作为无性系之间的鉴定依据。

ITS sequences（internal translation spacer） has been used to carry out difference analysis to identify GLGU-5,GLGU-9（two kinds of Eucalyptus grandis×E.urophylla elite clones）,GLU-4（one kind of E.urophylla elite clones） and seedlings of E.grandis×E.urophylla and E.urophylla.The experimental results were as follows： 1） The length range of ITS sequences of three elite clones was 38 to 45 bp longer than that in seedlings of E.grandis ×E.urophylla and E.urophylla,that proved the length difference ITS sequence could be used to identify these three tested elite clones and seedlings;2） There were ITS sequence differences among tested three elite clones,which could be used as the basis for identification of clone.