微波诱变在药源微生物菌株选育中的应用

Application of microwave mutagenesis in microorganism breeding for medicinal source strain improvement

微波诱变是一种较新兴非电离电磁辐射物理诱变育种技术,已开始应用于农作物、植物以及微生物育种研究中,并在药源微生物产能提高以及拓展活性菌株新资源相关研究中展露出很好的应用发展前景。本文结合本课题组新近研究进展,综述了微波的生物效应与诱变机制、诱变实验方法与影响诱变效果的因素、微波诱变技术在药源微生物菌株选育研究中的应用进展等,并对微生物微波诱变育种研究现状、优势和不足以及应用发展前景进行了讨论。

Microwave mutagenesis has become a new physical mutation technique by non-ionizing electromagnetic radiation, which has been used in the breeding of crops, plants and microorganisms, and has revealed bright future development in its application in the microorganism breeding for medicinal source strain improvement for providing high productivity or diverse bioactive mutants. This article reviews bioeffects of microwave, possible mechanisms of microwave mutagenesis, methods of microwave mutation technique together with main factors probably affecting microwave mutagenesis, and research advances in the application of microwave mutagenesis in microorganism breeding for medicinal source strain improvement. Meanwhile, the present research status, advantage and disadvantage, and prospect of this technique are also discussed focusing on its application in the microorganism breeding for medicinal source strain improvement.