苦参碱对人类肝癌细胞HepG2增殖、细胞周期及凋亡的影响

Effects of matrine on proliferation, cell cycle and apoptosis of human hepatocarcinoma cell line HepG2

目的探讨苦参碱（MT）对人类肝癌细胞株HepG2增殖、细胞周期及凋亡的影响。方法用不同质量浓度的MT（0．0125、0．025、0．05、0．1、0．2、0．4、0．8、1．6g／L）对培养的HepG2细胞作用不同时间（24、48、72h），用四氮噻唑蓝（MTT）比色法检测MT对细胞增殖的影响；流式细胞术（FCM）检测MT对HepG2细胞周期及凋亡的影响。结果质量浓度≥0．1g／L的MT有抑制HepG2细胞增殖的作用，且作用随药物质量浓度的增加及时间的延长而加强（P〈0．01）。FCM检测分析显示，0．8g／LMT作用48h能将HepG2细胞阻滞在G，期[（75.3±6．5）％对（64．1±6.3）％】（P〈0．05），1．6g／LMT作用48h能将HepG2细胞阻滞在G：期【（29．1±9．1）％对（11．6±2．1）％】（P〈0．01）。0．4、0．8、1．6g／LMT作用12、24、48h对HepG2细胞都有诱导凋亡作用，且作用随药物质量浓度的增加及作用时间的延长而加强（P〈0．01）。结论MT能够抑制HepG2细胞的增殖、诱导其凋亡并影响其细胞周期，呈时间和剂量依赖性。MT抗肝癌的机制可能与影响肝癌细胞周期、抑制细胞增殖及诱导凋亡有关。

Objective To study the effects of matrine on proliferation, cell cycle and apoptosis of human hepatocarcinoma cell line HepG2 and probe into the mechanisms of its anti-hepatocarcinoma effects. Methods The HepG2 cells were treated with different concentration of matrine （0.0125, 0.02, 0.05, 0.1, 0.2, 0.4, 0.8, 1.6 g/L） for different time （24, 48, 72 h）, then investigate the effects of matfine on cell proliferation by MTT, and the effects on cell cycle and apoptosis by flow cytometry. Results Matrine can inhibit the HepG2 cells proliferation at the concentration of 0.1 g/L and above in a concentration-dependent and time- dependent manner（P 〈0.01）. The result of FCM showed that the cell cycle of HepG2 was retarded at G1 phase treated with matfine for 48 h at the concentration of 0.8 g/L [（75.3±6.5）% vs （64.1±6.3）%, P 〈0.05], whereas was retarded at G2 phase treated with matrine for 48 h at the concentration of 1.6 g/L [（29.1±9.1）% vs （11.6±2.1）%, P 〈0.01]. The apoptosis of HepG2 cells can be induced by matrine for 12, 24 h or 48 h at the concentration of 0.4, 0.8, 1.6 g/L. Conclusion Matrine can inhibit cell proliferation, interfere cell cycle and inducing apoptosis of hepatocarcinoma cells, which may be involved in the mechanisms of matrine＇ s anti- hepatocarcinoma effects.