食管鳞状细胞癌组织微血管内皮细胞中巨噬细胞移动抑制因子蛋白的表达及其意义

Expression and significance of MIF protein in vascular endothelial cell in esophageal squamous cell carcinoma tissue

目的检测巨噬细胞移动抑制因子（MIF）蛋白标记的食管鳞状细胞癌（鳞癌）组织中的微血管密度（MVD），分析MVD与临床病理的相关性。方法用免疫组织化学的方法检测MIF蛋白在40例食管鳞癌组织微血管内皮细胞中的表达，计算MVD平均值并分析其与食管鳞癌临床病理的相关性。结果MIF蛋白在食管鳞癌组织微血管内皮细胞中的表达率为95．00％（38／40），与正常食管组织相比较[32．50％（13／40）】，两者差异有统计学意义（P〈0．05）。食管鳞癌组织MVD平均值为26．5±8．5，MVD与肿瘤的TNM分期、有无区域淋巴结转移密切相关（P〈0．01）。结论MIF可以作为食管鳞癌组织微血管内皮细胞的标志物之一，MVD与肿瘤的TNM分期、有无区域淋巴结转移密切相关，有可能成为预测食管鳞癌患者预后的主要因素之一。

Objective To detect the microvessel density（MVD） marked by MIF protein in esophagus squamous cell carcinoma（ESCC）, and to see if a relationship exists between microvessel density（MVD） and clinicopathologic features. Methods The expression of MIF in vascular endothelial cell in forty ESCC specimens was examined by immunohistochemistry staining, calculated the mean of MVD and analysed the relationship with clinicopathologic features. Results The positive rate of MIF protein was 95.00 %（38/40） in ESCC vascular endothelial cell. Compared with the normal esophageal squamous tissue[32.50 %（13/40）], there was significant difference in statistics, the mean of MVD was 26.5±8.5. MVD was positively correlated with the tumor TNM stages and lymph node . Conclusion MIF can be used in tagging vascular endothelial cell, MVD of ESCC was positively correlated with the tumor TNM stages and lymph node metastasis. It might be an major prognostic factor in ESCC.