食管癌患者外周血中自然杀伤性T细胞含量的检测及其临床意义

NKT cells in peripheral blood of patients with esophageal carcinoma and its clinical significance

目的研究食管癌患者外周血中自然杀伤性（NK）T细胞在手术前后的表达情况。方法采用流式细胞术（FCM）分析59例食管癌患者手术前后外周血中CD3、CD。CD56、CD8抗体的表达，研究NKT细胞及其亚群的表达情况及所占比例。结果随着CD3＋CD56＋CD8＋／CD；cD壶cD；的比值逐渐升高，Ⅲ～Ⅳ期食管癌患者的比例逐渐降低，ANOVA示组间差异有统计学意义（P〈0．05）；CD；CD；6CD；／CD；c聪CD；的比值与CD3＋CD56＋CD4＋成NKT细胞非线性相关；CD3＋CD56＋CD8＋；NKT细胞与NK细胞正相关，与CD3＋细胞负相关。结论CD3＋CD56＋CD8＋／CD3＋CD56＋CD4_的比值可能与肿瘤负荷有关，并且有助于判断食管癌患者的疾病程度及预后。

Objective To explore the expression, as well as the proportion of subsets of the natural killer （NK） T cells in peripheral blood of patients with esophageal carcinoma before and after surgery, and provide ideas and experimental basis for immune treatment through tests. Methods Using CD3, CD56, CD4 and CD8 antibody to study the NKT cells and its subsets. Peripheral NKT cell subsets in 59 patients with esophageal carcinoma were analysed by flow cytometer. Results With the ratio of CD; CD;6 CD＋/CD; CD56 CD8＋ gradually increased, the proportion of phase Ⅲ～Ⅳ patients with esophageal carcinoma is gradually decreased between the groups. One-way ANOVA analysis showed that there were differences between the groups （P 〈 0.05）. The ratio of CD3＋CD56＋CD8＋/CD3＋CD56＋CD4＋ was in Non-linear related to CD3＋CD;6 CD＋NKT cells; CD3＋ CD56＋CD8＋ NKT cells were positively correlated with CD3 CD56＋ NK cells and negatively correlated with CD; T cells. Conclusion The ratio of CD3＋CD56CD8＋/CD3＋ CD56＋ CD4＋ may relate to tumor burden, and is helpful to determine the extent of disease in patients with esophageal carcinoma and prognosis.