泽兰饮片及不同部位HPLC指纹图谱研究

HPLC fingerprint analysis of decoction pieces and different parts of Lycopus lucidus

目的:建立泽兰饮片的HPLC指纹图谱分析方法,并对泽兰茎、叶不同部位的指纹图谱进行比较。方法:采用Zorb-ax Esclipe XDB C18色谱柱;流动相为乙腈-0.1%磷酸(梯度洗脱);检测波长为330 nm;柱温为40℃;流速为1.0 mL/min。结果:泽兰共标示出18个共有峰,12批泽兰的相似度在0.957～0.999之间。但茎、叶指纹特征峰相对含量有显著差异,叶所含特征峰组分含量基本高于茎。结论:方法准确可靠,重复性好,为双柏制剂及泽兰饮片质量控制提供了参考。

AIM： To establish the method of fingerprint analysis on decoction pieces of Lycopus lucidus by HPLC and compare the variability of the different parts including stem and leaf.METHODS： HPLC was used with the Zorbax Esclipe XDB C18 column,the acetonitrile-0.1% phosphoric acid（gradient elution） was employed as a mobile phase,detection wavelength was at 330 nm,column temperature was at 40 ℃,and flow rate was 1.0 mL/min.RESULTS：Eighteen common peaks were separated on HPLC fingerprint in Lycopus lucidus.The similaritiesof 12 batches of samples were in the range of 0.957-0.999.There was significant variability between the stem andleaf.The marker peak in the leaf was more than that in the stem.CONCLUSION：The method is reliable andaccurate,has a better reproducibility,and provides a reference for the quality control over Shuangbai Powder anddecoction pieces of Lycopus lucidus.