星点设计-响应面法优化傣药竹叶兰总黄酮超声-双水相提取工艺

Ultrasound-assisted aqueous two-phase extraction of total flavonoids from Arundina graminifolia by central composite design and response surface method

目的:确定傣药竹叶兰中总黄酮物质的最佳提取工艺条件。方法:以芦丁为指标应用超声耦合丙醇-硫酸铵双水相体系提取竹叶兰中总黄酮物质,采用星点设计-响应面分析法优化提取工艺条件,考察丙醇浓度、硫酸铵用量、超声时间3个因素对竹叶兰总黄酮得率的影响。结果:竹叶兰总黄酮最佳提取条件:丙醇浓度为55%,超声时间为50 min,硫酸铵用量20%,总黄酮得率为3.53%。结论:双水相体系对竹叶兰总黄酮有较好的提取作用,工艺简单、省时、易操作、易于放大。

AIM： To obtain the optimal extraction conditions of general flavonoids from Arundina graminifolia Dai nationality folk medicine.METHODS： The rutin was assigned to marker,the ultrasonic-assisted propanol in combination with（NH4）2SO4 aqueous two-phase system were used to extract the total flavonids from Arundina graminifolia.With three factors including propanol concentration,quantity of（NH4）2SO4 and ultrasonic process time,by central composite design and response surface method.RESULTS： The optimum extraction condition was composed of 55% of propanol concentration 50 min ultrasonic time,and 20% of ammonium sulfate dosage.Under this condition,the yield was up to 3.53%.CONCLUSION： The experiment shows that ultrasonic coupling with propanol-ammonium sulfate aqueous two-phase system is an effective method for extracting the general flavonoids from Arundina graminifolia.The method is simple,convenient and easily enlarging.