益气活血法对关节炎大鼠滑膜细胞凋亡及血清细胞因子的影响

The Influence of the Therapy for Nourishing Qi and Activating Circulation on Synovial Cell Apoptosis and Serum Cytokines in Rheumatoid Arthritis Rats

目的:选用黄芪与川芎嗪注射液配伍(简称配伍组),观察"益气活血法"对佐剂性关节炎(AA)大鼠滑膜细胞凋亡及血清细胞因子的影响,探索该治则治法治疗类风湿性关节炎(RA)的作用靶点和作用机理。方法:建立AA大鼠模型,设立正常组、模型组、黄芪组、川芎嗪组、配伍组、甲氨喋呤(MTX)及雷公藤多苷(TPT)组进行实验研究,观察益气、活血及配伍后对AA大鼠足跖肿胀的影响,对滑膜细胞凋亡及血清细胞因子浓度的影响,阐释其作用机制。结果:配伍组能显著提高滑膜细胞的凋亡率,与MTX、TPT组效果相当;能显著抑制注射足跖肿胀程度,并降低大鼠血清中IL-1、TNF-α、IL-6的水平,上调IL-4的浓度,抑制免疫性炎症,优于MTX、TPT。结论:益气活血法可能通过促进AA大鼠滑膜细胞的凋亡,抑制某些炎性因子的释放,起到治疗RA的作用,体现了中医治则治法指导下中药复方配伍的优势。

Objective： To observe the effect of Astragalus root and Chuanxiong rhizome used in combination（called combination treatment group） on synovial cell apoptosis and serum cytokines in Rheumatoid Arthritis Rats and explore the mechanism of the therapy for nourishing qi and activating circulation in treating RA.Methods： AA rat model was established.The rats were divided into seven groups：normal,model,Astragalus root treatment,Chuanxiong rhizome treatment,combination treatment,MTX treatment and TPT treatment.Observe the influence and mechanism of the therapy for nourishing qi and activating circulation to the swelling of vola pedis,synovial cell apoptosis ratio and density of serum cytokines in AA rats.Results： Combination treatment group could distinctly recede the swelling of vola pedis,heighten synovial cell apoptosis ratio and decrease the content of IL-1、TNF-αand IL-6 in serum,but the content of IL-4 in serum was increased,rather and even superior to MTX and TPT.Conclusion： The mechanism of the therapy for nourishing qi and activating circulation in treating RA could be to restrain inflammation cytokines and decreasing the synovial cell apoptosis of AA rats,which reflect the advantages of Chinese medicine prescription.under the direction of the treatment principles and methods of TCM.