紫外-可见分光光度法测定蓝花参中多炔类化合物的含量

Quantitative Determination of Total Polyactetylenes in Wahlenbergia marginata (Thunb.) A.DC. by UV Spectrophotometry

目的:建立蓝花参提取物中多炔类化合物含量测定方法。方法:以党参炔苷为指标成分,对蓝花参提取物中多炔类化合物采用乙醇回流提取,采用紫外-可见分光光度法测定其含量。结果:多炔类化合物在4～28μg/mL范围内线性良好,精密度、稳定性、重复性和加样回收率均达到要求。结论:该实验方法简便、快捷、结果可靠。

Objective：To determine the total polyactetylenes in Wahlenbergia marginata（Thunb.） A.DC..Method：Using detection wavelength and lobetyolin as the index component,the polyacetylenes substances in Wahlenbergia marginata（Thunb.） A.DC.extract were determined through ethanol reflux extraction and ultraviolet spectrophotometer.Results：Sample size showed a good linear realationship at the range of 4～28μg/mL,precision,stability,repeatability and recovery are achieved.Conclusion：This method is easy,rapid and reiliable.