水稻小孢子发生过程中微管骨架的初步研究

A Preliminary Study on Microtubular Cytoskeleton During Microsporogenesis of Rice

以正常水稻品种IR36和光温敏核雄性不育系培矮64S为材料，采用间接免疫荧光标记技术，观察了小孢子发生过程中微管构型的变化。两份材料均形成了前期的核周微管、中期的纺锤体微管和末期的成膜体微管等类型。但减数分裂前期不育系培矮64S的部分小孢子母细胞微管构型表现出一些细微变化，微管排列紊乱，粘成柬状。推测这些变化可能与部分小孢子的败育有关．

By using the technique of indirect immunofluorescence labeling, some changes of microtubular configuration in the microsporogenesis were observed in IR36, a fertile normal cultivar andPeiai 64S, a photoperiod and temerature sensitive genic male sterile line. All types of microtubulearrays were formed in the two materials, namely, interphase or prophase perinuclear microtubule,metaphase spindle microtubule and anaphase phragmoplast microtubule, etc. Fine variations, however, were detected in distribution pattern of some Peiai 64S microsporocyte microtubule. Microtubules were disorderly arranged and sticking bundle. It is tentatively proposce that microtubulardistribution variations are perhaps associated with some microspores abortion.