鸽Ⅰ型禽副粘病毒F基因克隆及序列分析

Cloning and Sequencing of F Gene from the Pigeon' Avian Paramyxovirus-Ⅰ

利用RT－PCR技术获得了编码鸽Ⅰ型禽副粘病毒GD／P1分离株F0裂解位点附近884bp的F基因cDNA片段，并将其克隆到pGEM－TEasy质粒中，获得了重组质粒T－pPWV－F－I，核酸序列测定和分析表明，该片段与新城疫病毒强毒株F48E9和HER／33相应区域的同源性分别为87．5％和88．19％，与新城疫病毒弱毒株LaSota和B1株的同源性分别为83．83％和84．15％．该病毒F0裂解位点的氨基酸序列为Arg－Arg－Gln－Lys－Arg－Phe－Ile－Gly－Ala．这在分子水平上证明该毒株较接近于新城疫病毒强毒。

In this study, a 884 bp cDNA fragment encoding the cleavage site of F0 protein, from a pigeon-derived avian paramyxovirus-Ⅰ isolate called strain GD/P1, was got by using RT-PCR. The fragment was cloned into pGEM-T Easy vector. Yielding the recombinant plasmid-T-pPMV-F-I. After sequencing, it was found that the nucleotide sequence showed 87. 5%, 84. 15%, 83. 83% and 84. 15%homology with the newcastle disease virus (NDV) virulent strains (F48E9 and HER/33 ) and avirulentstrains (LaSota and B1) respectively. The dedveted amino acid sequence at the cleavage site of F0 proteinwas Arg- Arg- Gln-Lys - Arg- Phe - Ile - Gly - Ala . It concluded that this virus was closly related to the NDV virulent strains.