小麦成株期高温抗条锈基因Yrxy54-1的RGAP分子标记定位

Molecular mapping of high-temperature adult-plant stripe rust resistance gene Yrxy54-1 with RGAP markers

采用我国当前流行的7个小麦条锈菌(菌系),在高温条件下对其进行成株期抗条锈性鉴定,并用条锈菌CYR32对小麦品种小偃54与铭贤169的杂交后代及其双亲进行高温成株期抗条锈性遗传分析,以揭示小偃54成株期抗条锈性遗传机制。结果显示,小偃54成株期对多个条锈菌小种具有良好的抗病性,对CYR32的抗病性由2对隐性核基因独立控制,分别暂命名为Yrxy54-1和Yrxy54-2,并对Yrxy54-1进行分子标记定位。从528个RGAP引物组合中筛选到4个与抗病基因Yrxy54-1紧密连锁的多态性标记M1、M2、M3和M4,分布于Yrxy54-1的两侧,遗传距离分别为15.8、16.6、7.3和9.97cM。遗传分析结合分子标记结果表明,Yrxy54-1是一个与已知抗条锈基因不同的新基因。

To reveal mechanism of resistance to stripe rust in Xiaoyan54,which was inoculated with seven dominant races or strains of Puccinia striiforms f.sp. tritici prevalent in China,Xiaoyan54,susceptible cultivar Mingxian169 and their cross combinations were tested with races CYR32. RGAP markers were used to locate the resistance genes. The results showed that Xiaoyan54 expressed good disease resistance to seven dominant races or strains of PST at the adult stage. Genetic analysis of Xiaoyan54 indicated that resistance gene conferring to CYR32 was controlled by two independent recessive genes,temporarily designated as Yrxy54-1 and Yrxy54-2,respectively,and Yrxy54-1 was located. The parents,resistant and susceptible bulks were used for screening 528 RGAP primer combinations. The markers,M1,M2,M3,M4,were tightly linked to Yrxy54-1. Genetic distance from M1,M2,M3,and M4 to Yrxy54-1 were 15.8,16.6,7.3,and 9.97 cM,respectively. Genetic analysis of Yrxy54-1 combined with RGAP markers supported the conclusion that Yrxy54-1 was a novel resistant gene which was different from other stripe rust resistance genes.