RP-HPLC法测定生脉注射液中五味子醇甲的含量

Content Determination of Schizandrin in Shengmai Injection by RP-HPLC

目的:建立生脉注射液中五味子醇甲含量的测定方法。方法:采用反相高效液相色谱(RP-HPLC)法。色谱柱为DikmaO测D浓S度C1(8在135.02～mm64×4m.6g·mLm-(,1r5=μ0m.)99,9流9动,n相=为6)甲范醇围-内水与(6峰5:面35积),积检分测值波线长性为关25系0n良m,好流;速高为、中1.、0低mL浓·度mi的n-加1。样结回果收:五率味为子9醇8.2甲4%的～检100.93%,RSD<2.0%。结论:RP-HPLC法测定五味子醇甲的含量稳定可靠,重复性好,可作为生脉注射液的质量控制方法。

OBJECTIVE：To establish RP-HPLC method for the content determination of Schizandrin in Shenmai injection.METHODS：The detection was performed on Dikma C18（150 mm×4.6 mm,5 um） column with methanol-water（65：35） as mobile phase at a flow rate of 1.0 mL·min-1 and UV detection wavelength was set at 250 nm.RESULTS：The linear range of schizandrin was 3.2-64 mg·L-1（r=0.999 9,n=6） with recovery of 98.24%-100.93%（RSD2.0%）.CONCLUSION：The method is stable,reliable and reproducible for quality control of Shengmai injection.