线性扫描伏安法测定头孢拉定胶囊中头孢拉定的含量

Determination of Cefradine in Cefradine Capsules by Linear Sweep Voltammetry

目的:建立线性扫描伏安法(LSV)和循环伏安法(CV)等电化学方法测定头孢拉定胶囊含量的方法。方法:工作电极为多壁碳纳米管修饰玻碳电极(MWCNT/GCE),参比电极为Ag/AgC(l饱和KCl溶液)电极、辅助电极为铂丝电极。结果:在优化试验条件下,还原峰电流与头孢拉定浓度在5.0×10-6～1.0×10-4mol·L-1范围内呈良好的线性关系(r=0.9973),检测限为1.7×10-6mol·L-1,平均加样回收率为99.8%,RSD=1.02%(n=9)。结论:该方法简单、快速、灵敏,可用于头孢拉定各种剂型的质量分析。

OBJECTIVE：To develop linear sweep voltammetry（LSV） and cyclic voltammetry（CV） for the content determination of Cefradine capsules.METHODS：Multi-wall carbon nanotube modified glassy carbon electrode（MWCNT/GCE） was used as working electrode,Ag/AgCl（saturated KCl solution） electrode was used as reference electrode and supporting electrode was platinum electrode.RESULTS：Under the optimized experimental conditions,the reduction peak current varying linearly with the concentration of cefradine ranged from 5.0×10-6 to 1.0×10-4 mol·L-1（r=0.997 3） with a detection limit of 1.7×10-6 mol·L-1.The average recovery was 99.8%（RSD=1.02%,n=9）.CONCLUSION：The method is simple,rapid and sensitive and can be used for the content determination of cefradine in various dosage forms.