软骨源抑制因子抑制角膜新生血管的实验研究

An experimental study of anti angiogenesis with a cartilage derived inhibitor

目的观察软骨源抑制因子（ｃａｒｔｉｌａｇｅｄｅｒｉｖｅｄｉｎｈｉｂｉｔｏｒ，ＣＤＩ）在活体状态下对角膜新生血管的抑制效果。方法制备高纯度的ＣＤＩ和４组（６４只眼）的兔角膜新生血管模型，采用角膜微袋分析技术和病理方法定量检测，观察在不同情况下ＣＤＩ对角膜新生血管的抑制作用。结果纯化的ＣＤＩ对角膜新生血管的生长速度和生长面积均有显著的抑制作用（Ｐ＜０．００１）。结论纯化的ＣＤＩ在一定条件下能特异性地抑制机体组织中的角膜新生血管生长，抑制效果呈剂量依赖性。

Objective To evaluate the efficacy of a cartilage derived inhibitor for the treatment of corneal neovascularization in vivo. Methods A cartilage derived inhibitor (CDI) from bovine scapula was purified to homogeneity. By rabbit corneal neovascularization(CNV) model, the effect of inhibition of CDI under various conditions was determined in corneal micropocket analysis. Results The purified CDI could inhibit strongly the growing speed and area of rabbit CNV compared to control ( P <0.001). Conclusion It is demonstrated that CDI is a potent dose dependent inhibitor of angiogenesis.