大鼠骶尾腹内侧肌的运动神经分布——CB-HRP、CB-胶体金结合CGRP免疫组织化学双标法研究

EFFECT OF TRIPTERYGIUM WILFORDII HOOK ON NERVE REGENERATION AFTER HETEROGRAFTING

目的通过研究Ｏｎｕｆ核靶肌肉的神经分布特点，探讨在某些运动神经元疾病中，Ｏｎｕｆ核及其靶肌肉功能不受影响的生理机制。方法应用ＣＢ－ＨＲＰ逆行示踪技术及ＣＢ－胶体金逆行示踪结合ＣＧＲＰ免疫组织化学双标技术，观察了支配大鼠骶尾腹内侧肌的运动神经元在脊髓内的定位分布。结果首次发现该肌的神经分布方式与会阴肌的神经分布相同，即同时接受脊髓前角躯体运动神经元和Ｏｎｕｆ核神经元的双重支配。其中有６３．４０％位于Ｌ６～Ｓ３前角的腹侧群，２２．３０％位于Ｏｎｕｆ核的背外侧群，１４．３０％位于Ｏｎｕｆ核的背内侧群。各核群内支配该肌的神经元约５０％为ＣＧＲＰ阳性神经元。另外，支配该肌的上述两种运动神经元在Ｌ６～Ｓ３节段软脊膜处特定部位形成丰富的软脊膜下树突丛。结论实验证实了骶尾腹内侧肌不但是Ｏｎｕｆ核最大的靶肌肉，而且支配该肌的运动神经元具有丰富的软脊膜下树突丛。

Objective\ In the present experiment,the effect of tripterygium wifordii hook on nerve regeneration in heterograft was explored.Methods\ The rats were given tripterygium wilfodii hook preparation(C 6H 13(14) O 6) after nerve grafting from rabbits (Japanese big ear stain,as donor)to rats(Wistar,as recipient). Results\ At 4,8,12,16 and 24 weeks after grafting,it was found that regenerating nerve fibers grew into the graft nerve and extended to the distal nerve segment.In the graft nerve and distal nerve segment,both myelinated and unmyelinated fibers of regenerating nerve were seen.Most of them were arranged in fascicles.Twelve weeks after grafting,gastrocnemius muscle showed positive AChE reaction and the regenerating nerve fibers connected with motor endplates.The regenerating nerve fascicles in subcutaneous tissus of the claws and the free nerve endings in derma were seen.In the control group without Tripterygium wilfordii hook,no nerve regeneration occurred due to the immune rejection. Conclusion\ The Tripteryguim wilfordii hook might play a role of immunodepression in the nerve heterografting.