参麦注射液对百草枯所致肺损伤的保护作用

Protective effect of ginseng and ophiopogon injection on pulmonary damage caused by paraquat poisoning

目的探讨参麦注射液在百草枯中毒所致肺损伤中的作用。方法120只SD大鼠随机分为百草枯中毒组（APP），参麦注射液治疗组（SM1，SM2，SM3）。HE染色观察参麦注射液治疗组组织结构变化，采用免疫组织化学法测定各组肺组织中TGF—β1、IL-1β的表达水平。结果百草枯中毒组肺组织充血水肿明显，肺泡破坏严重，治疗组有所减轻；各治疗组与百草枯中毒组之间转化生长因子β-1（TGF—β1），白细胞介素-1β（IL—1β）水平均较治疗组明显降低，各治疗组随剂量的增加，TGF-β1，IL-1β水平有明显下降，差异有显著性（P〈0．05）。结论参麦注射液在百草枯所致中毒大鼠肺损伤中能使TGF—β1，IL-1β表达水平下调，减轻肺泡炎症和充血程度，减轻百草枯中毒所致肺损伤，延缓和抑制肺纤维化的发生、发展。

Objective To investigate the effects of ginseng and ophiopogon （Shenmai Injection, SM injection） in treatment of acute paraquat poisoning （APP）. Methods One hundred and twenty Wister rats were randomly divided into 4 equal groups： APP model group, undergoing gastric peffusion of paraquat 80 mg/kg, SM1 group undergoing intraperitoneal injection of SM injection 3.6 ml/kg onee a day for 7 d, SM2 group undergoing intraperitoneal injection of SM injection 7.2 ml/kg once a day for 7 d, and SM3 group undergoing intraperitoneal injection of SM injection 14.4 ml/kg once a day for 7 d. The rats were killed at days 7 and 28 at random with their lungs taken out to undergo pathological examination. Immunohistochemical methods were used to detrect the transforming growth factor-β 1 （TGF-β 1） and interleukin-1β（IL-1 β）. Results Obvious alveolotis was seen in the APP group, and the alveolotis was milder, and the higher SM dose the milder the inflammation. The intensity levels of TGF-β1 and IL-1β of the APP group were high. SM injection dose- and time-dependently decreased the TGF-β 1 and IL-1β levels. Conclusion SM injection is an effective medicine to treat pulmonary fibrosis induced by APP.