重度烧伤患者细胞因子水平的变化及其临床意义

The clinical change of cytokines expression in severely burned patients

目的:通过研究重度烧伤患者体内细胞因子水平的变化,初步探讨其与疾病严重程度的关系及其临床意义。方法:选择烧伤面积≥35%烧伤总面积(TBSA)的患者与正常对照组(与烧伤组平行处理)各49例,分别收集烧伤患者伤后6 h、12 h、1天、2天、7天、14天的外周静脉血。ELISA试剂盒检测细胞因子IL-2、IL-4、IL-6、IL-8的分泌水平;CCK-8试剂盒检测T淋巴细胞增殖能力。结果:与正常对照组比较,重度烧伤患者伤后6 h、12 h、1天、2天、7天、14天T淋巴细胞增殖能力与IL-2、IL-4的分泌水平均显著下降(P均<0.05),而IL-6、IL-8的分泌水平则显著升高(均P<0.05)。结论:重度烧伤患者体内T淋巴细胞免疫功能处于抑制状态,促炎细胞因子(IL-6、IL-8)参与烧伤后炎症应答的病理生理过程。

Objective： To investigate the clinical change of cytokines expression in severely burned patients.Methods：Forty-nine burned patients with burn surface exceeding 35% TBSA and healthy controls of same number were enrolled in this study.The peripheral blood of patients were collected at 6 h,12 h and 1,2,7,14 post-burn days.The T lymphocyte proliferation ability was analyzed by CCK-8 and the levels of IL-2、IL-4、IL-6、IL-8 in patients and healthy donors were detected by ELISA.Results：Compared with that in healthy controls,the proliferation ability of T lymphocyte and the levels of IL-2,IL-4 were significantly decreased in patients on 6 h,12 h and 1,2,7,14 post-burn days（all P 〈 0.05）,but the levels of IL-6,IL-8 were significantly higher than those of healthy controls（both P 〈 0.05）.Conclusion：The immunological function of T lymphocyte in severely burned patients is depressed persistently,and the pro-inflammatory cytokines could participate in the pathological process of inflammatory response following burn.