鼻咽泡状核细胞癌和低分化鳞癌的临床生物学行为的对比研究

A COMPARATIVE STUDY OF VESICULAR NUCLEUS CELL CARCINOMA AND POORLY DIFFERENTIATED SQUAMOUS CELL CARCINOMA IN NASOPHARYNX

目的：探讨鼻咽泡状核细胞癌（ＶＮＣＣ）和低分化鳞癌（ＰＤＳＣＣ）的临床生物学行为的差异性。方法：用原位杂交和免疫组化染色（ＳＰ法）技术，检测ＶＮＣＣ和ＰＤＳＣＣ癌组织中ＣｅｒｂＢ２、表皮生长因子受体（ＥＧＦＲ）和增殖细胞核抗原（ＰＣＮＡ）表达，用原位末端标记（ＩＳＬＥ）方法检测癌细胞凋亡，并对其预后和临床特征进行对比分析。结果：①ＶＮＣＣ的ＩＳＥＬ和ＰＣＮＡ染色强度指数（ＳＩ）明显高于ＰＤＳＣＣ；②ＶＮＣＣ的ＣｅｒｂＢ２、ＥＧＦＲｍＲＮＡ和ＣｅｒｂＢ２蛋白表达与ＰＤＳＣＣ无明显差别；③ＶＮＣＣ的５年生存率比ＰＤＳＣＣ高。结论：与ＰＤＳＣＣ比较，ＶＮＣＣ是一种生长增殖活跃、分化程度更低，但有较好预后趋向的一种癌型。

Objective: To compare the oncogene expression, the cell proliferation and apoptosis, and the clinical features of vesicular nucleus cell carcinoma (VNCC) and poorly differentiated squamous cell carcinoma (PDSCC) in nasopharynx. Methods: C erbB 2, and epidermal growth factor receptor (EGFR), and proliferating cell nuclear antigen (PCNA) expressed in VNCC and PDSCC were detected by in situ hybridization and immunohistochemical staining. Apoptosis of cancer cells was examined by in situ end labelling (ISLE) techniques. Results: VNCC showed higher PCNA staining intensity index (SII), and higher cell apoptosis SII, and a higher five year survival rate in comparision with PDSCC. But there was no difference of the expression of C erbB 2, EGFR genes between VNCC and PDSCC. Conclusion: VNCC is a more poorly differentiated carcinoma, but has better prognosis than PDSCC.