多肿瘤抑制基因—p16在喉癌中的丢失研究

DELETION OF MULTIPLE TUMOR SUPPRESSOR GENE(p16) IN LARYNGEAL SQUAMOUS CELL CARCINOMA

目的：探讨ｐ１６基因的缺失与人喉癌的发生、发展的关系。方法：应用ＲＴ－ＰＣＲ及免疫组化方法检测ｐ１６。结果：４４例喉癌患者中２３例存在ｐ１６的失活，失活率为５２．３％，ｐ１６基因的失表达与喉癌的组织学分化程度及临床分期有密切关系（Ｐ＜０．０５），表明ｐ１６基因的变异有可能与人喉癌的发生发展有关，提示检测ｐ１６基因的丢失对于判断喉癌的恶性度及估计病情的进展具有重要意义。结论：随着对ｐ１６基因的不断了解，将为肿瘤的临床诊断、基因治疗提供新的思路和线索。

Objective: To investigate the role of p16 gene aberrant alteration in pathogenesis and progression of human larynx carcinoma.44 cases of larynx carcinoma were collected in Tianjin District between October 1996 and September 1997. Methods: RT PCR and immunohistochemical methods were used to examine p16 gene expression. Results: p16 deletion existed in 23 cases; the deletion rate was 52.3%. Conclution: p16 non expression has close relation with differentiation degree and clinical stages of larynx carcinoma.It is suggested that examination of p16 deletion is significant for the judgement of the malignancy and estimation of the progress of larynx carcinoma.