表皮生长因子、碱性成纤维细胞生长因子及5-氟尿嘧啶对培养的人视网膜色素上皮损伤愈合的体外研究

Effects of epidermal growth factor, basic fibroblast growth factor and 5 fluorouracil on human retinal pigment epithelial wound closure in vitro

目的定量观察表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）、碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）及５氟尿嘧啶（５ｆｌｕｏｒｏｕｒａｃｉｌ，５Ｆｕ）对人视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ，ＲＰＥ）细胞迁移与增殖能力的影响，探讨ＥＧＦ、ｂＦＧＦ及５Ｆｕ在增殖性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｖｉｔｒｅｏｒｅｔｉｎｏｐａｔｈｙ，ＰＶＲ）形成及防治中的作用。方法应用人ＲＰＥ细胞损伤模型、细胞增殖曲线法，在倒置相差显微镜下观察人ＲＰＥ细胞在各种条件作用下的损伤愈合与增殖过程。结果在ＥＧＦ、ｂＦＧＦ作用下，ＲＰＥ细胞分别以迁移方式或扩散方式覆盖创面，前者使ＲＰＥ细胞胞体延长呈纺锤形，细胞间隙明显加宽；后者使ＲＰＥ细胞大而扁平呈梭形，细胞紧密排列。两者虽可加快ＲＰＥ细胞损伤愈合过程，但其作用甚微。５Ｆｕ可明显降低ＲＰＥ细胞增殖能力，但不影响其迁移功能。结论ＥＧＦ、ｂＦＧＦ单独应用对创伤愈合作用不明显。这两种生长因子在ＰＶＲ中对ＲＰＥ细胞形态的影响可能与ＲＰＥ细胞的转化密切相关。５Ｆｕ仅能作为一种?

Objective To study the effects of epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and 5 flurouracil (5 Fu) on the cell migration and proliferation. Methods Phase contrast microscopy, cell proliferation assay and wound closure were used to study the process of cell proliferation and wound healing in a model of human retinal pigment epithelial (RPE) cell damage. Results It was found that EGF mainly stimulated proliferation and the migration of indvidual RPE cells from the wound edge, and induced cellular elongation, while bFGF mainly promoted proliferation and spread of the confluent monolayer into the wound defect, and induced enlargement and flattening of cells. But the effects of EGF, bFGF on wound closure were not very significant ; 5 Fu inhibited the spread and proliferation of RPE cells, but had no effects on cell migration. Conclusions EGF and bFGF have no significant effects on wound healing, perhaps their effects on cell morphology are very important in cell transformation in proliferative vitreoretinopathy (PVR), and 5 Fu can only be a subsidiary drug to prevent PVR.