摘要
目的定量观察表皮生长因子(epidermalgrowthfactor,EGF)、碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)及5氟尿嘧啶(5fluorouracil,5Fu)对人视网膜色素上皮(retinalpigmentepithelium,RPE)细胞迁移与增殖能力的影响,探讨EGF、bFGF及5Fu在增殖性玻璃体视网膜病变(proliferativevitreoretinopathy,PVR)形成及防治中的作用。方法应用人RPE细胞损伤模型、细胞增殖曲线法,在倒置相差显微镜下观察人RPE细胞在各种条件作用下的损伤愈合与增殖过程。结果在EGF、bFGF作用下,RPE细胞分别以迁移方式或扩散方式覆盖创面,前者使RPE细胞胞体延长呈纺锤形,细胞间隙明显加宽;后者使RPE细胞大而扁平呈梭形,细胞紧密排列。两者虽可加快RPE细胞损伤愈合过程,但其作用甚微。5Fu可明显降低RPE细胞增殖能力,但不影响其迁移功能。结论EGF、bFGF单独应用对创伤愈合作用不明显。这两种生长因子在PVR中对RPE细胞形态的影响可能与RPE细胞的转化密切相关。5Fu仅能作为一种?
Objective To study the effects of epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and 5 flurouracil (5 Fu) on the cell migration and proliferation. Methods Phase contrast microscopy, cell proliferation assay and wound closure were used to study the process of cell proliferation and wound healing in a model of human retinal pigment epithelial (RPE) cell damage. Results It was found that EGF mainly stimulated proliferation and the migration of indvidual RPE cells from the wound edge, and induced cellular elongation, while bFGF mainly promoted proliferation and spread of the confluent monolayer into the wound defect, and induced enlargement and flattening of cells. But the effects of EGF, bFGF on wound closure were not very significant ; 5 Fu inhibited the spread and proliferation of RPE cells, but had no effects on cell migration. Conclusions EGF and bFGF have no significant effects on wound healing, perhaps their effects on cell morphology are very important in cell transformation in proliferative vitreoretinopathy (PVR), and 5 Fu can only be a subsidiary drug to prevent PVR.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1999年第2期134-136,I006,共4页
Chinese Journal of Ophthalmology
关键词
色素上皮
视网膜病变
EGF
BFGF
5-FU
Pigment epithelium, eye Epidermal growth factor urogastrone Fibroblast growth factor, basic Fluorouracil Wound healing