外周前庭器官损伤后传出性前庭神经系统降钙素基因相关肽的变化

Calcitonin gene related peptide changes in efferent vestibular system during vestibular compensation

目的探讨外周前庭器官损伤后不同时间病理状态下传出性前庭神经系统降钙素基因相关肽（ｃａｌｃｉｔｏｎｉｎｇｅｎｅｒｅｌａｔｅｄｐｅｐｔｉｄｅ，ＣＧＲＰ）的变化。方法制作ｗｉｓｔａｒ大鼠单侧外周前庭损伤模型２９只，对照健康动物１６只，取动物脑干切片，用ＡＢＣ法作传出性前庭神经系统ＣＧＲＰ免疫组化反应。结果健康动物传出性前庭神经系统ＣＧＲＰ呈弱阳性反应，单侧前庭损伤后双侧传出性前庭神经系统ＣＧＲＰ反应呈强阳性反应，其阳性反应程度在损伤后１０～１２ｄ达高峰，以后随前庭功能恢复而逐渐减弱，但５个月时阳性反应程度仍高于健康动物。结论传出性前庭神经系统ＣＧＲＰ在外周前庭器官损伤后可能起一定的调节性作用。

Objective To investigate the calcitonin gene related peptide (CGRP) effect on efferent vestibular system during pathological state of vestibular afferent system.Methods An animal model of vestibular compensation was made by administration of streptomycin to rat vestibular organ to destroy unilateral vestibular function . Change of CGRP in efferent vestibular neurons in all processes from vestibular disorder to vestibular compensation was observed utilizing ABC method of immnuohistochemical technique.Results Efferent vestibular neurons of normal animals showed low immunoreactivity to CGRP. The number of CGRP immunoreactivity neurons and level of CGRP immunoreactivity increased in efferent vestibular system during vestibular disorder, and these changes decreased with vestibular compensation.Conclusion Activity of CGRP in efferent vestibular system plays a regular role on accelerating vestibular compensation.