PDGF-A及bFGF表达与移植血管平滑肌细胞增殖的关系

Relationship between PDGF A,bFGF and proliferation of smooth muscle cells in vein grafts

目的探讨血小板源性生长因子Ａ（ＰＤＧＦＡ）和碱性成纤维细胞生长因子（ｂＦＧＦ）与移植血管平滑肌细胞增殖的关系。方法建立３２只大鼠自体静脉移植模型，分别于术后６小时、４８小时、１周、２周、４周切取移植静脉，应用免疫组织化学动态观察不同时期移植血管ＰＤＧＦＡ及ｂＦＧＦ表达情况。结果ＰＤＧＦＡ的变化在时间上与血管平滑肌细胞（ＳＭＣ）增殖活性的变化是相对平行的：均于术后逐渐增加，于１周达高峰，与术后６小时相比差异有极显著意义（Ｐ＜００１）。ｂＦＧＦ分别于术后２天及２周出现两个高峰，与其他时间组相比差异均有显著性意义（Ｐ＜００５）。结论ＰＤＧＦＡ对移植静脉ＳＭＣ迁移和增殖起重要作用，ｂＦＧＦ除对ＳＭＣ增殖起促进作用外，而且可能参与内皮化过程。

Objective To detect the relationship between platelet derived growth factor A (PDGF A),basic fibroblast growth factor (bFGF), and proliferation of smooth muscle cells in vein grafts. Methods An animal model of autogenous vein graft was established by transplanting internal branch of the jugular vein to the common iliac artery by end to end anastomosis. 25 rats were used. The grafted veins were harvested at 6 h, 2 d, 1 w, 2 w and 4 w respectively after the operation. The expression of PDGF A and bFGF in different stages after grafting procedure was observed immunohistochemically. Results The expression of PDGF A peaked at 1 w, paralleled with proliferation of SMC, and was significantly higher than that at 6 h( P <0 01).The expression of PDGF A diminished gradually. At 4 w, it was significantly lower than that at 1 w ( P <0 01). bFGF demonstrated bimodal pattern of the expression: the first peak of expression occurred by 2 d and the second peak by 2 w. The difference was significantly compared with other stages. Conclusions The expression of PDGF A 1 contributed to migration and proliferation of SMC. Except for contributing to proliferation of SMC, bFGF perhaps took part in endothelization.