脂质体-C-erbB2反义脱氧寡核苷酸对人卵巢癌裸鼠网膜移植瘤生长及化疗药物敏感性的影响

Effect of Liposome C erbB2 Antisense Oligodeoxynucleotides on the Growth and the Chemotherapeutic Drug Sensitivity of Human Ovarian Cancer Transplanted in the Omentum of Nude Mice

目的探讨脂质体ＣｅｒｂＢ２反义脱氧寡核苷酸（ＣｅｒｂＢ２ＳＯＤＮｓ）对人卵巢癌裸鼠网膜移植瘤生长及顺铂（ＤＤＰ）敏感性的影响。方法对２０只裸鼠建立人卵巢癌裸鼠网膜移植瘤模型，分为对照组（腹腔注射无菌水）、观察组（腹腔注射脂质体ＣｅｒｂＢ２ＳＯＤＮｓ）、化疗组（腹腔注射无菌水及ＤＤＰ）、观察＋化疗组（腹腔注射脂质体ＣｅｒｂＢ２ＳＯＤＮｓ及ＤＤＰ）。治疗期间观测裸鼠体重变化，治疗结束后取瘤体称重进行电镜观察。结果观察组抑瘤率为３７％，电镜下可见细胞异染色质增多，线粒体退化。观察＋化疗组抑瘤率可达５０％。观察组裸鼠体重无明显改变。结论ＣｅｒｂＢ２反义基因治疗在卵巢癌基因治疗中有一定作用，与化疗联合应用可以取得更好的治疗效果。

Objective To investigate the effects of liposome C erbB2 antisense phosphorothioate oligodeoxynucleotides(S ODNs)on the growth and the chemotherapeutic drug sensitivity of human ovarian cancer transplanted in the omentum of nude mice. Methods Human ovarian cancer transplanted in the omentum of nude mice model was established, then divided into four groups: control group, experimental group, chemotherapeutic group, and experimental +chemotherapeutic group. Different treatments were given respectively. The weight of nude mice was observed and the morphology of tumor cells was observed by electromicroscope. Results The growth inhibitory rate in the experimental group was 37%. There were more heterochromatins in the treated tumor cells. The growth inhibitory rate in the experimental+ chemotherapeutic group was increased to 50%. There was no obvious alteration in the weight of experimental group. Conclusion The data in this study suggest that antisense therapy is an useful method of gene therapy in ovarian cancer;Moreover, it could enhance the effectiveness of antitumor drug.