外周血来源内皮祖细胞自体移植对油酸诱发兔急性肺损伤的影响

Effect of autologous transplantation of circulating endothelial progenitor cells on oleic acid-induced acute lung injury in rabbits

目的 探讨外周血来源内皮祖细胞（EPC）自体移植对油酸诱发兔急性肺损伤的影响.方法 雄性新西兰大白兔30只,体重1.8～2.0 kg,随机分为2组（n=15）：生理盐水对照组（NS组）和EPC自体移植组（EPC组）.经右耳缘静脉缓慢注射油酸80 mg/kg制备急性肺损伤模型,注射油酸后4 h时EPC组经耳缘静脉注射200μl生理盐水（含自体EPC 106个）,NS组注射等容积生理盐水.于注射油酸前（T0）和注射油酸后4、8、12、24、48 h（T1-5）时抽取耳中央动脉血行血气分析,计算氧合指数,采用ELISA法测定血清血管内皮细胞生长因子（VEGF）浓度;于T5时采集血样后处死动物取肺,测定肺含水量、中性粒细胞浸润率、肺出血和透明膜面积.结果 与NS组比较,EPC组T3-5时氧合指数和T4-5时血清VEGF浓度升高,T5时肺含水量、中性粒细胞浸润率、肺出血面积和透明膜面积降低（P＜0.05或0.01）.结论 外周血来源EPC自体移植可减轻油酸诱发兔的急性肺损伤.

Objective To investigate the effect of autologous transplantation of circulating endothelial progenitor cells （EPCs） on oleic acid-induced acute lung injury （ALI） in rabbits. Methods Thirty New Zealand long ear rabbits weighing 1.8-2.0 kg were randomly divided into 2 groups （ n = 15 each）： normal saline group （group NS） and EPC group. ALI was induced by iv oleic acid 80 mg/kg. EPC （106/200 μl） or equal volume of normal saline （NS） was administered iv at 4 h after iv oleic acid injection. Arterial blood samples were obtained before （T0） and at 4, 8, 12, 24 and 48 h （T1-5） after oleic acid injection for blood gas analysis and determination of serum vascular endothelial growth factor （VEGF） concentration. The animals were then scrificed （at T5 ） and the lungs were removed for microscopic examination and determination of W/D lung weight ratio, Pulmonary infiltration of PMN and non-PMN was counted and hyaline membrane formation and hemorrhage were examined. Results PaO2/FaO2 ratio and serum VEGF concentration were significantly higher in group EPC than in group NS. Infiltration of leukocytes in the lung was significantly reduced by EPC transplantation. EPC also decreased lung water content, hyaline membrane formation and hemorrhage in the lungs. Conclusion Autologous transplantation of circulating EPC can ameliorate oleic acid-induced acute lung injury in rabbits.