芳香化酶细胞色素P450在子宫内膜异位症中的检测与临床意义

The Expression and Significance of Aromatase Cytochrome P450 in Endometrium from Women with Endometriosis

目的:探讨芳香化酶细胞色素P450(P450arom)在子宫内膜异位症(EM)患者子宫内膜中的表达及临床意义。方法:采用免疫组织化学方法检测63例EM患者、40例妇科良性疾病患者及30例正常对照组患者子宫内膜中P450arom蛋白的表达,并结合EM患者的临床分期等进行比较分析。结果:P450arom蛋白在正常对照组子宫内膜中无表达;在良性疾病对照组中阳性检出率为17.50%(7/40),表达水平(0.22±0.16);在EM患者子宫内膜中阳性检出率为81%,表达水平(0.57±0.26),差异有统计学意义(P<0.05)。P450arom蛋白表达在增生期与分泌期差异无统计学意义(P>0.05)。P450arom蛋白表达随EM临床分期增加呈增强趋势,但差异无统计学意义(r=0.226,P>0.05)。结论:P450arom在EM患者子宫内膜中的高表达与EM的发生有关;检测P450arom蛋白在子宫内膜的表达可作为诊断EM新的参考指标。

Objective：To investigate the expression and clinical significance of aromatase cytochrome P450（P450arom） in endometrium of endometriosis patients.Methods：The expression of P450arom was examined by immunohistochemistry in endometrium of 63 patients with endometriosis,40 patients with benign gynecologic diseases and 30 normal control women.Results：The positive expression rate of P450arom was 0 in normal endometrium tissues,17.50% in tissues of benign gynecologic diseases（0.22±0.16） and 80.95% in endometriosis with the expression level of 0.57±0.26（P 0.05）.The expression of P450arom was not related to the menstrual periods,which were proliferative phase and secretory phase（P 0.05）.The expression of P450arom was enhanced with the clinical stage,but there was no statistic significance（r = 0.226,P 0.05）.Conclusion：The higher-expression of P450arom in endometrium was related to the development of endometriosis.The expression of P450arom may be one of parameters for understanding the endometriosis.