肝硬化形成过程中大鼠肝组织组织胺1、2受体mRNA表达的变化

Change in histamine-1 and-2 receptors mRNA expression during the development of experimental rat liver cirrhosis

目的研究肝硬化形成过程中大鼠肝组织组织胺1受体（H1R）和组织胺2受体（H2R）mRNA表达的变化。方法用内对照逆转录定量聚合酶链反应（RT-PCR）对经四氯化碳诱导的肝硬化形成过程中2周、4周、6周、8周（各12只）和对照组大鼠（12只）肝组织H1R和H2R的mRNA表达进行研究。结果H1R的mRNA水平：对照组（2．308±0．202）显著高于肝硬化形成过程中的2周（0．756±0．130）、4周（O．999±0．207）、6周（1．127±0．217）、8周（1．564±0．130）（P＜0．01），肝硬化各组间差异有显著性（P＜0．05）。H2R的mRNA水平：对照组（0．851±0．208）显著高于肝硬化形成过程中的2周（0．263±0．104）、4周（0．244±0．092）、6周（0．225±0．047）、8周（0．243±0．062）（P＜0．01），肝硬化形成过程各组间差异无显著性（P＞0．05）。结论大鼠肝组织以H1RmRNA的表达为主，肝硬化形成过程中肝细胞上的H1R和H2RmRNA的表达明显减少。

Objective To investigate the changes in histamine-1 receptor (H1R) and histamine-2receptor (H2R) mRNA expression in the liver tissues of the rats during the development of experimentalcirrhosis. Method Reverse transcription polymerase chain reaction (RT-PCR) was used to study theH1R and H2R mRNA expression in liver tissues in 12 control rats and 48 experimental rats (n= 12 in 2,4, 6, 8 weeks each respectively) with CC14-induced cirrhosis. Results Expression of H1R mRNA in thehepatic tissues of the control rats was 2. 308±0. 202, significantly higher than in the experimental rats(P<0. 01 ). Significant difference in H1R mRNA expression was found among the cirrhosis groups (P<0. 05). Expression of H2R mRNA in the hepatic tissues of the control rats was 0. 851±0. 208,marked1y higher than in the cirrhosis groups (P<0. 01 ). Conclusions H1R mRNA in the rat hepatictissues was mainly expressed. The expression of H1R and H2R mRNA in the hepatic tissues of the experimental rats was decreased significantly.