粘性放线菌菌毛生物性能的研究 Ⅱ.粘性放线菌菌毛化学组成的分析

A Study on Biological Properties of Fimbriae of Actinomyces viscosus Ⅱ.Chemical Characteristics of Fimbriae of A.viscosus

目的：了解采用机械搅拌法提取的粘性放线菌Ⅰ型、Ⅱ型两种菌毛的化学组成。方法：首先制备出抗Ⅰ型菌毛抗血清和抗Ⅱ型菌毛抗血清，鉴定两种菌毛纯化物纯度后，采用考马斯亮蓝法和蒽酮法分别测定两种菌毛中蛋白质和糖的含量，并进行氨基酸组成分析。结果：两种菌毛的主要成分是蛋白质，糖含量较低。两种菌毛均是天门冬氨酸、谷氨酸、丙氨酸、赖氨酸和甘氨酸含量较多。两种菌毛中，非极性氨基酸百分含量最高，碱性氨基酸百分含量最低。结论：本实验结果有助于了解两种菌毛的化学组成与其生物性能的关系。

Objective: To investigate the chemical charateristics of two purified fimbriae. Methods: The specific antibodies against type Ⅰ and type Ⅱ fimbriae of A.viscosus were prepared and the purity of the two purified fimbriae had been identified by the specific antibodies. Then, chemical analysis and amino acid analysis of the two purified fimbriae were done. Results: The two purified fimbriae were mainly composed of proteins and small amount of carbohydrate. The two types of fimbriae contained large amounts of aspartic acid, glutamic acid, alanine, lysine and glycine, and high percentage of nonpolar amino acid was analysed while basic amino acids were present in a very small amounts. Conclusion: These results may help to understand the relationship between the chemical characteristics of two types of fimbriae and their biological activity.